Cereal Crops Research Articles

Change in Cultivation of Bajra in Haryana and Rajasthan

Background: Bajra (Pennisetum glaucum) is the main coarse cereal crop grown mainly in Africa and Asia. In India, it is mainly grown in Rajasthan and Haryana. It is cultivated in the dry and warm climatic regions. It is a Kharif crop that is grown at the start of the season’s first monsoon. Objective: This study aimed to explore the change in the cultivation of bajra in two states, Haryana and Rajasthan. Cultivation means how the crop is grown in any particular area and what steps are taken to improve the growth of crops. Methods: In this study, different aspects like area, productivity, and yield were studied from 2018 to 2020, and data were compiled for all these factors in both states with the help of various literature research. Results: This study demonstrated that the growth trends in the area under cultivation, productivity, and yield of bajra increased from 2018 to 2020 due to stability in yield, price (MRP), and insured marketing. All the results and data were obtained from different published sources in these two states. Conclusion: This review gives a brief account of the changes in the cultivation of bajra in two different states, Haryana and Rajasthan. The expansion of the area of bajra is due to the use of good quality seeds, the use of hybrids, and the adaption of good agricultural practices, and it is also the reason for an increase in production and yield.

Cytokinin: An invisible warrior to spot blotch disease: A new proposal to way forward

Background of Abstract: Wheat is threatened by spot blotch disease causing considerable yield loss, with existing genotypes lacking resistance and the necessary nutrients. Cytokinins (CKs) are key phytohormones that not only regulate plant development but also play an important role during spot blotch disease and in the nutrient metabolic pathway of crop plants. Body of Abstract: Spot blotch disease, caused by Bipolaris sorokiniana syn. Helminthosporium sativum syn. Cochliobolus sativus is one of the dangerous diseases of wheat (Triticum aestivum L.) in Asia. Previous study identified the significant impact of zinc (Zn), sulfur(S), iron (Fe), nitrogen(N), potassium(P), and chlorine (Cl) fertilization on dipping the spot blotch acuteness in two wheat varieties Bhrikuti and Sonalika in Nepal. Cytokinins (CKs) are crucial phytohormones that not only control plant growth but also show a significant function during the nutrient metabolic pathway and in the stressed condition of the cereal crop plants. Lack of vital nutrients like Zn, S and Fe initiates severe injury of the body which is pleading the urgent requirement to improve the increase of these micronutrients in the cereal crop plant. Crop bio-fortification is one of the promising methods through which these nutrients could be boosted to a desirable volume. Cytokinin has a crucial role in controlling environmental stress and defense methods of plants. It is also discovered that it has an effect over Zn growth in cereal crop plants. Many studies identified that an augmentation of Zn, S and Fe in the seeds of the cereal crop plants ensuing the improvement of the root elongation which is also helpful for foraging nutrients to the innate ends of the soil and supports in the water absorbance in the drought area. Traveling micronutrients from the lithosphere through the root supports in the uptake of the micronutrients and transferring them to the cereal crop plants. Short Conclusion: This review is exploring a crop bio- fortification method containing CRISPR-Cas9 through breeding methods could be a significant job for spot blotch stress management and developing the desirable traits to boost the nutritional rate of wheat which would be beneficial for mankind.

Recycling Waste Agricultural Nets as Cement Composites

The advancement of agricultural mesh technology has contributed to its improved properties. As a result, agricultural nets are widely adopted in large-scale farming applications, for example, in cereal crop farming. However, a consequence of this increased use of agricultural nets is the accumulation of large amounts of waste. The current paper focuses on the recycling of agricultural nets used in wrapping straw bales to develop additives and fillers in cement composites. The research details an analysis of the use of waste agricultural meshes as an ingredient in cement composites. Six test series of different mixtures were conducted. In the first four series, agricultural waste was utilised as an additive in a composite comprising aggregate and cement slurry (the amounts of wasted nets were 20, 40, 60, and 80 kg/m3). In the last test series, the recyclate utilised comprised a mixture of cement slurry and waste only. The composites were subjected to standard tests and thermal resistance tests. The results showcased that that the addition of a net worsened the workability of the concrete mixture, and with increasing amounts of addition, the consistency of the mixture could change from liquid to dense plastic. The flexural strength of the composite decreased with increasing amounts of recyclate. In subsequent test series, the flexural strength value was lower than that of the control (3.93 MPa), from 7.38% (3.64 MPa) for the composite with 20 kg/m3 of recyclate to 37.66% (2.45 MPa) for the composite with of 80 kg/m3 recyclate. The flexural strength value of the net-filled composite without aggregate was very high (10.44 MPa), where the value obtained for the control composite was 62.36% lower. The results of the compressive strength test showed a decrease in this parameter with increasing amounts of additive. The value assessed for the control composite was 27.99 MPa. As expected, the composite that had no aggregate and consisted of only recycled filler had the lowest compressive strength. The value of this parameter was 13.07 MPa, and it was 53.31% lower than that of the control composite. The results of the tests of resistance to temperatures were similar to those recorded for the composites with polypropylene fibres. All composites demonstrated a significant decrease in their compressive and flexural strength after annealing. SEM imaging showed that the net fibres were closely bonded to the cement stone. Finally, it was concluded that recyclates performed best as fillers in lightweight composites with a low density, low absorption, high flexural strength, and satisfactory compressive strength.

Guard cell and subsidiary cell sizes are key determinants for stomatal kinetics and drought adaptation in cereal crops.

Climate change-induced drought is a major threat to agriculture. C4 crops have a higher water use efficiency (WUE) and better adaptability to drought than C3 crops due to their smaller stomatal morphology and faster response. However, our understanding of stomatal behaviours in both C3 and C4 Poaceae crops is limited by knowledge gaps in physical traits of guard cell (GC) and subsidiary cell (SC). We employed infrared gas exchange analysis and a stomatal assay to explore the relationship between GC/SC sizes and stomatal kinetics across diverse drought conditions in two C3 (wheat and barley) and three C4 (maize, sorghum and foxtail millet) upland Poaceae crops. Through statistical analyses, we proposed a GCSC-τ model to demonstrate how morphological differences affect stomatal kinetics in C4 Poaceae crops. Our findings reveal that morphological variations specifically correlate with stomatal kinetics in C4 Poaceae crops, but not in C3 ones. Subsequent modelling and experimental validation provide further evidence that GC/SC sizes significantly impact stomatal kinetics, which affects stomatal responses to different drought conditions and thereby WUE in C4 Poaceae crops. These findings emphasize the crucial advantage of GC/SC morphological characteristics and stomatal kinetics for the drought adaptability of C4 Poaceae crops, highlighting their potential as future climate-resilient crops.

CRISPR/Cas9-mediated editing of GmDWF1 brassinosteroid biosynthetic gene induces dwarfism in soybean.

The study on the GmDWF1-deficient mutant dwf1 showed that GmDWF1 plays a crucial role in determining soybean plant height and yield by influencing the biosynthesis of brassinosteroids. Soybean has not adopted the Green Revolution, such as reduced height for increased planting density, which have proven beneficial for cereal crops. Our research identified the soybean genes GmDWF1a and GmDWF1b, homologous to Arabidopsis AtDWF1, and found that they are widely expressed, especially in leaves, and linked to the cellular transport system, predominantly within the endoplasmic reticulum and intracellular vesicles. These genes are essential for the synthesis of brassinosteroids (BR). Single mutants of GmDWF1a and GmDWF1b, as well as double mutants of both genes generated through CRISPR/Cas9 genome editing, exhibit a dwarf phenotype. The single-gene mutant exhibits moderate dwarfism, while the double mutant shows more pronounced dwarfism. Despite the reduced stature, all types of mutants preserve their node count. Notably, field tests have shown that the single GmDWF1a mutant produced significantly more pods than wild-type plants. Spraying exogenous brassinolide (BL) can compensate for the loss in plant height induced by the decrease in endogenous BRs. Comparing transcriptome analyses of the GmDWF1a mutant and wild-type plants revealed a significant impact on the expression of many genes that influence soybean growth. Identifying the GmDWF1a and GmDWF1b genes could aid in the development of compact, densely planted soybean varieties, potentially boosting productivity.

Long-term expansion of cereal crops promotes regional population increase of polyphagous Helicoverpa armigera

Long-term expansion of cereal crops promotes regional population increase of polyphagous Helicoverpa armigera

Analysis of the Management of Cereal Crop Residues on Farms to Optimise Cattle Feed in the Western Cotton Growing Zone of Burkina Faso

Crop residues are insufficiently valorised by farmers in western Burkina Faso, while cattle face a shortage of forage. The aim of this study was to analyse the management of cereal crop residues in cotton-cereal farming to optimise their use as feed for draught cattle. A sample of 72 cotton farmers was selected from nine villages in the study area by the stratification method and interviewed using a semi-structured questionnaire. The quantities of cereal crop residue biomass collected by farmers were assessed directly in the field. The results showed that maize and sorghum stalks were predominantly used in cattle feed (86.1 and 90.3%), composting (50 and 44.4%), and mulching (26.9 and 25%), respectively. The average quantities of cereal crop residues stored were 0.83, 0.16, and 1.19 tonnes of dry matter (tDM) for crop farmers, livestock breeders, and agro-pastoralists, respectively, with respective fodder balances of -3.99, -29.05, and -16.99 Tropical Cattle Units (TCU) for the use of cattle, or -0.88, -0.38, and -1.81 TCU for the use of draught cattle during the lean season. Concentrated feed use was 0.20, 0.05, and 0.21 kg/cattle/day; respectively. The main factors influencing the quantities of cereal residues stored on the farms were the size of the cultivated areas and the farmers’ capacity for transportation. Provision of transport equipment and storage infrastructure would therefore appear to be a solution that could improve the level of fodder residue storage, particularly for draught oxen.

Metabolomic Profile and Functional State of Oat Plants (Avena sativa L.) Sown under Low-Temperature Conditions in the Cryolithozone

Oats are one of the most useful and widespread cereal crops in the world. In permafrost conditions (Central Yakutia), based on metabolic changes in late summer-sown oat plants (Avena sativa L.), the key processes involved in the cold acclimation of a valuable cereal species were identified. During the onset of low ambient temperatures, metabolites from leaf samples were profiled using gas chromatography with mass spectrometry (GC-MS) and were analyzed using principal component analysis (PCA). A total of 41 metabolites were identified in oat leaves. It was found that acclimation to suboptimal temperatures during the fall period leads to biochemical (accumulation of mono- and disaccharides and decrease in fatty acids and polyols) as well as physiological and biophysical changes (decrease in leaf PRI reflectance indices and chlorophyll a fluorescence). Therefore, the study contributes to a more holistic understanding of oat metabolism under low-temperature cryolithozone stress. It is believed that the analysis of changes in leaf reflection properties and JIP-test parameters of chlorophyll a fluorescence using leaf metabolomic profiling can be used in the selection of valuable varieties of cereal crops to obtain plant fodders with high nutrient contents under conditions of a sharply continental climate.

Are cereal grasses a single genetic system?

In 1993, a passionate and provocative call to arms urged cereal researchers to consider the taxon they study as a single genetic system and collaborate with each other. Since then, that group of scientists has seen their discipline blossom. In an attempt to understand what unity of genetic systems means and how the notion was borne out by later research, we survey the progress and prospects of cereal genomics: sequence assemblies, population-scale sequencing, resistance gene cloning and domestication genetics. Gene order may not be as extraordinarily well conserved in the grasses as once thought. Still, several recurring themes have emerged. The same ancestral molecular pathways defining plant architecture have been co-opted in the evolution of different cereal crops. Such genetic convergence as much as cross-fertilization of ideas between cereal geneticists has led to a rich harvest of genes that, it is hoped, will lead to improved varieties.

Comparing various bio-intensive pest management modules in rice

Rice is the key cereal crop in the tropics, supporting diverse pests and natural enemy populations. Recurrent pest outbreaks and yield losses led farmers to rely on extensive insecticide application, disturbing the stability of rice ecosystems and increasing residues in the harvestable products. A study has been conducted to assess the impact of various modules of Bio-Intensive Pest Management (BIPM) on its conservational potential and sustainability in comparison with Farmers’ Practices (FP) in Nalgonda district of Telangana state during kharif-2020. The BIPM practices included application of farm yard manure; rice husk ash; clipping of seedlings; alleyways and weekly release of Trichogramma japonicum in the main field; wet seed treatment and foliar application with Pseudomonas fluorescens (BIPM 1), Trichoderma asperellum strain TAIK1 (BIPM 2) and Bacillus cabrialesii strain BIK3 (BIPM 3). Whereas, FP 1 with need-based insecticide spraying and FP 2 with schedule-based insecticide spraying and Untreated control with (UC) with no intervention. The observations were taken by visual counts at 15-day intervals. The incidence of Cnaphalocrocis medinalis was highest in UC (9.50), followed by BIPM 1 (7.25) and least in FP 2 (2.50) whereas the highest mean population of Apanteles sp. was found in the untreated control (13.75), followed by BIPM 3 (9.50) and least in FP 2 (4.25). The Benefit Cost Ratios (BCR) of BIPM 3 (1.68) were highest followed by BIPM 1 (1.64) and least in FP 2 (1.40) elucidating that BIPM practices can be more economically feasible. The Shannon-Wiener Index for species diversity and species evenness was higher in BIPM treatments as compared to farmers’ practices indicating the potential of these BIPM treatments in natural pest control and maintaining crop ecosystem stability.

Exogenous application of salicylic acid ameliorates salinity stress in barley (Hordeum vulgare L.)

Barley (Hordeum vulgare L.) is a significant cereal crop belonging to Poaceae that is essential for human food and animal feeding. The production of barley grains was around 142.37 million tons in 2017/2018. However, the growth of barley was influenced by salinity which was enhanced by applying a foliar spray of salicylic acid. The current study investigated to evaluated the potential effect of SA on the barley (Hordeum vulgare L.) plants under salinity stress and its possible effects on physiological, biochemical, and growth responses. The experiment was conducted at Postgraduate Research Station (PARS), University of Agriculture; Faisalabad to assess the influence of salicylic acid on barley (Hordeum vulgare L.) under highly saline conditions. The experiment was conducted in a Completely Randomized Design (CRD) with 3 replicates. In plastic pots containing 8 kg of properly cleaned sand, two different types of barley (Sultan and Jau-17) were planted. The plants were then watered with a half-strength solution of Hoagland’s nutritional solution. After the establishment of seedlings, two salt treatments (0 mM and 120 mM NaCl) were applied in combining three levels of exogenously applied salicylic acid (SA) (0, 0.5, and 1 mg L-1). Data about morphological, physiological, and biochemical attributes was recorded using standard procedure after three weeks of treatment. The morpho-physiological fresh weight of the shoot and root (48%), the dry mass of the shoot and root (66%), the plant height (18%), the chlorophyll a (30%), the chlorophyll b (22%), and the carotenoids (22%), all showed significant decreases. Salinity also decreased yield parameters and the chl. ratio (both at 29% and 26% of the total chl. leaf area index). Compared to the control parameters, the following data was recorded under salt stress: spike length, number of spikes, number of spikelets, number of tillers, biological yield, and harvest index. Salicylic acid was used as a foliar spray to lessen the effects of salinity stress, and 1 mg L-1 of salicylic acid proved more effective than 0.5 mg L-1. Both varieties show better growth by applying salicylic acid (0 mg L-1) as a control, showing normal growth. By increasing its level to (0.5 mg L-1), it shows better growth but maximized growth occurred at a higher level (1 mg L-1). Barley sultan (Hordeum vulgare L.) is the best variety as compared to Jau-17 performs more growth to mitigate salt stress (0mM and 120mM NaCl) by improving morpho-physiological parameters by enhancing plan height, Root and shoot fresh and dry weights, as well as root and shoot lengths, photosynthetic pigments, area of the leaves and their index, and yield attributes and reduce sodium ions.

A Genome-Wide Association Study of Seed Morphology-Related Traits in Sorghum Mini-Core and Senegalese Lines

Sorghum (Sorghum bicolor L.) ranks fifth as the most crucial cereal crop globally, yet its seed morphology remains relatively unexplored. This study investigated seed morphology in sorghum based on 115 mini-core and 130 Senegalese germplasms. Eight seed morphology traits encompassing size, shape, and color parameters were assessed. Statistical analyses explored potential associations between these traits and resistance to three major sorghum diseases: anthracnose, head smut, and downy mildew. Furthermore, genome-wide association studies (GWAS) were conducted using phenotypic data from over 24,000 seeds and over 290,000 publicly available single nucleotide polymorphisms (SNPs) through the Genome Association and Prediction Integrated Tool (GAPIT) R package. Significant SNPs associated with various seed morphology traits were identified and mapped onto the reference sorghum genome to identify novel candidate defense genes.

Анализ климата и сельскохозяйственных факторов для прогнозирования урожайности основных зерновых культур в Эфиопии: визуальный анализ (1995-2021 гг.)

<p>This paper serves as a foundational exploration into predicting the yields of important cereal crops in Ethiopia. We use a visual analytic approach to identify patterns in annual temperature, precipitation, area harvested, production, and yield data from 1995 to 2021 by integrating climate factors and agricultural practices. By examining these Agricultural variables, we hope to build links between shifting climatic conditions, agricultural decisions, and crop production, providing indispensable insights for stakeholders, farmers, and policymakers.</p>

Major transcription factor families at the nexus of regulating abiotic stress response in millets: a comprehensive review.

Millets stand out as a sustainable crop with the potential to address the issues of food insecurity and malnutrition. These small-seeded, drought-resistant cereals have adapted to survive a broad spectrum of abiotic stresses. Researchers are keen on unravelling the regulatory mechanisms that empower millets to withstand environmental adversities. The aim is to leverage these identified genetic determinants from millets for enhancing the stress tolerance of major cereal crops through genetic engineering or breeding. This review sheds light on transcription factors (TFs) that govern diverse abiotic stress responses and play role in conferring tolerance to various abiotic stresses in millets. Specifically, the molecular functions and expression patterns of investigated TFs from various families, including bHLH, bZIP, DREB, HSF, MYB, NAC, NF-Y and WRKY, are comprehensively discussed. It also explores the potential of TFs in developing stress-tolerant crops, presenting a comprehensive discussion on diverse strategies for their integration.

Is food self-sufficiency possible for Reunion Island?

In the context of political and economic instability, food self-sufficiency of countries and territories is becoming a burning issue. Reunion Island is a densely populated small French territory isolated in the middle of the Indian Ocean. The previously developed GRAFS model, allowing consistent balances to be established using nitrogen (N) as a common metric for all crops and foodstuffs, has been applied to Reunion Island, considering 11 sub-regions to account for landscape variety. Reunion Island dedicates 87% of its crop production in terms of harvested proteins to the exportation of sugar and tropical fruits, while it imports 67% of its food supply, 54% of livestock feed, and 57% of all fertilizing N inputs to agricultural soils. Overall, the supply of 1 tonne of N as food requires the import of 2.7 tonnes of N as food, feed, and fertilizers. The model also demonstrated that the simultaneous operation of three levers of change would make it possible to reach self-sufficiency in terms of food, feed, and fertilizer: (1) the generalization of agro-ecological crop rotations alternating grain and forage legumes, cereal, and other food crops; (2) the reconnection of livestock with crop farming and a better recycling of manure as well as of human excreta; (3) a drastic reduction of animal-based food in the Reunionese diet, down to 20% of animal products in the total per capita protein intake, instead of the 60% current share. The area dedicated to sugarcane cultivation should be reduced to 15–25% of its current value.

Environmental Impact Assessment of Organic Wheat Cracker Value Chains with and without Nettle Powder as a Natural Additive: A Case of Sweden

Due to the growing global population and consequent increased demand for food, the global production of cereal crops has increased. Wheat is one of the most important food crops in the world, as its products, e.g., bread and crackers, have served as important sources of nutrition for many years. However, the environmental impacts of wheat-derived food products are not frequently explored. This study presents an environmental impact assessment of organic wheat crackers within the context of Swedish winter wheat production using both primary and literature-based data. A cradle-to-consumer gate life cycle analysis (LCA) approach using the functional unit (FU) of 1 kg of crackers was applied while considering two cracker value chains: (i) without additives and (ii) using nettle powder as a natural additive. Four environmental impact categories—cumulative energy demand (CED), climate change impact (GWP), acidification, and eutrophication—were explored, with a particular focus on CED and GWP. The analysis results indicated that the total CED values were about 13 MJ/FU and 14 MJ/FU for crackers without and with the additive, respectively. Similarly, the total GWP values were 379 g CO2 eq/FU and 464 g CO2 eq/FU, respectively. The post-harvest processing and handling stage was an environmental hot spot in both cases. The introduction of the nettle additive has increased the quantified values of all four of the investigated impact categories. These insights will enable food processors and policy makers to communicate the environmental impacts and make informed decisions to improve the sustainability of wheat crackers. This paper contributes to a database of the environmental impacts of wheat products, specifically LCA data of organic wheat crackers and the LCA method for further LCA studies of snacks and other wheat products with plant-based functional additives.

Meta-QTL analysis reveals the important genomics regions for biotic stresses, nutritional quality and yield related traits in pearl millet

AbstractPearl millet (Cenchrus americanus) is the sixth most significant cereal crop cultivated on 30 million ha and a staple diet for 90 million poor people across the globe. Besides abiotic stresses several biotic stresses have been limiting production of pearl millet in the semi-arid and arid regions. Although, the Quantitative Trait Loci (QTLs) associated with key diseases like blast, rust and downy mildew resistance and nutritional content has been reported, the use of these QTLs is limited in breeding programs. To identify highly stable consensus genomic regions, we conducted Meta-QTL analysis using 191 QTLs reported in 12 independent studies over the last two decades. As a result, we report 34 Meta-QTLs regions on a consensus genetic map comprising of 692 markers and spanning 2070.7 cM. The confidence interval of Meta-QTLs was reduced by 3.63 folds (0.18–7.49 cM), in contrast to projected QTLs interval of 1.11–60.63 cM. Further, a total of 1198 genes were identified in 34 Meta-QTL regions. Among 34 Meta-QTL regions, Meta-QTL1.1 is found to be region of significant importance as it harbours genes for enhanced biotic stress tolerance, plant growth and development as well as genes related with enhanced seed development. Meta-QTL2.4 has highest number of genes with a significant role in disease resistance which contains basic leucine zipper domain, zinc family, leucine rich repeat regions. Meta-QTL3.1 has ABC transporter like activity coupled with the ATPase activity which has a role in Fe and Zn uptake in leaves and root tissues. These Meta-QTL regions can be used in genomics-assisted breeding for enhancing the blast, rust downy mildew resistance as well as yield and nutritional traits.

Mapping the race between crop phenology and climate risks for wheat in France under climate change.

Climate change threatens food security by affecting the productivity of major cereal crops. To date, agroclimatic risk projections through indicators have focused on expected hazards exposure during the crop's current vulnerable seasons, without considering the non-stationarity of their phenology under evolving climatic conditions. We propose a new method for spatially classifying agroclimatic risks for wheat, combining high-resolution climatic data with a wheat's phenological model. The method is implemented for French wheat involving three GCM-RCM model pairs and two emission scenarios. We found that the precocity of phenological stages allows wheat to avoid periods of water deficit in the near future. Nevertheless, in the coming decades the emergence of heat stress and increasing water deficit will deteriorate wheat cultivation over the French territory. Projections show the appearance of combined risks of heat and water deficit up to 4years per decade under the RCP 8.5 scenario. The proposed method provides a deep level of information that enables regional adaptation strategies: the nature of the risk, its temporal and spatial occurrence, and its potential combination with other risks. It's a first step towards identifying potential sites for breeding crop varieties to increase the resilience of agricultural systems.

Integrated risk assessment and human health hazards via dietary intake of OCPs from cereal crops cultivated across river chenab

The current study aimed to employ integrated risk assessment models to estimate carcinogenic and non-carcinogenic human health risks of organochlorine pesticides (OCPs) from Pakistan. GIS-based geostatistical technique was used to classify OCPs' polluted risk zones using risk indexes. The residual level of ∑OCPs detected in wheat and rice ranged from 1.78 to 12.46 ng g−1 and 4.92–18.19 ng g−1, respectively with the prevalence of DDD, DDE and HCH. The distribution pattern revealed a significantly higher concentration towards downstream region, suggesting industrial and agricultural activities as a pollution source in the area. Single pollution index (SPI) was employed for the identification of pollution zones. The findings of SPI for detected OCPs were found (0.7 < Pi < 1), respectively, depicting minimal pollution at the studied sites. Furthermore, risk assessment results reflected non-cancer risk (>1) for studied ∑OCPs. However, the suggested USEPA value for tolerable carcinogenic risk is < 10−6 (one in a million) which was found higher for DDD, DDE, HCH, CT, and HC in the current study. Consequently, the carcinogenic risk in the study area indicated the need for an assessment, monitoring, and reporting program grounded on laws that can reduce pollution and exposure levels to pesticides in Pakistan for good health and well-being.

The Egyptian wheat cultivar Gemmeiza-12 is a source of resistance against the fungus Zymoseptoria tritici

BackgroundWheat is one of the world’s most important cereal crops. However, the fungal pathogen Zymoseptoria tritici can cause disease epidemics, leading to reduced yields. With climate change and development of new agricultural areas with suitable environments, Z. tritici may advance into geographical areas previously unaffected by this pathogen. It is currently unknown how Egyptian wheat will perform in the face of this incoming threat. This project aimed to assess the resistance of Egyptian wheat germplasm to Z. tritici, to identify cultivars with high levels of resistance and characterise the mechanism(s) of resistance present in these cultivars.ResultsEighteen Egyptian wheat cultivars were screened against two Z. tritici model isolates and exhibited a wide spectrum of responses. This ranged from resistance to complete susceptibility to one or both isolates tested. The most highly resistant cultivars from the initial screen were then tested under two environmental conditions against modern UK field isolates. Disease levels under UK-like conditions were higher, however, symptom development on the cultivar Gemmeiza-12 was noticeably slower than on other Egyptian wheats. The robustness of the resistance shown by Gemmeiza-12 was confirmed in experiments mimicking Egyptian environmental conditions, where degree of Z. tritici infection was lower. The Kompetitive allele-specific PCR (KASP) diagnostic assay suggested the presence of an Stb6 resistant allele in several Egyptian wheats including Gemmeiza-12. Infection assays using the IPO323 WT and IPO323ΔAvrStb6 mutant confirmed the presence of Stb6 in several Egyptian cultivars including Gemmeiza-12. Confocal fluorescence microscopy demonstrated that growth of the IPO323 strain is blocked at the point of stomatal penetration on Gemmeiza-12, consistent with previous reports of Stb gene mediated resistance. In addition to this R-gene mediated resistance, IPO323 spores showed lower adherence to leaves of Gemmeiza-12 compared to UK wheat varieties, suggesting other aspects of leaf physiology may also contribute to the resistance phenotype of this cultivar.ConclusionThese results indicate that Gemmeiza-12 will be useful in future breeding programs where improved resistance to Z. tritici is a priority.